How HGP Was Done/The Process?

To start off in order to sequence a whole genome you don’t need to know whole base pair,because as the matches for base pairs are always same(Adenine and Timine,Cytosine and Guanine).Also,considering a human genome approximately has 3 billion base pairs,it is not a quick process.But considering EVERYTHING we have can be found on our genome,there is no wonder why it is that complicated

            To sequence our genome,scientists took samples from some volunteers and then removed the information papers on sampling tubes(containing name,age,gender etc.)Scientists call sequencing technique as BAC(Bacterial Artificial Chromosome).Whole genome has been fragmented to smaller pieces(between 150.000-200.000 base pairs for each group).All fragments were cloned in a bacteria in order to replicate and sequence them.About 20.000 BACs were used for mapping whole human genome.After that,each group gets cut into even smaller groups(about 2.000 bases in length) and those little groups are where sequencing reaction happens.Later on their products are sent to a sequencer which which sequences each base about 10 times and these bases represent human DNA in a BAC clone.Than computer programs are used to read the genome map and look at the results and analyze them.